A researcher wants to compare how much myosin protein is present in samples of fish muscle tissue. All muscle tissue contains myosin. Muscle tissue from three types of fish, salmon, catfish, and trout, are prepared and loaded into separate wells on a polyacrylamide gel. Electrophoresis is conducted and the gel is stained with Coomassic blue. Which statements below are truc (select all that apply). a. Only one single blue band corresponding to mysoin protein will be present in each lane. b. Each lane will contain multiple blue bands representing the array of proteins present in fish muscle tissue. No bands will be visible unless the proteins are transferred to a blot, probed with antibody specific for myosin, and subjected to colorimetric detection. d. Multiple protein bands will be visible after the proteins are transferred to a blot, probed with myosin antibody, and subjected to colorimetric detection.

Answers

Answer 1

Based on the information provided, the correct statements are Each lane will contain multiple blue bands representing the array of proteins present in fish muscle tissue and Multiple protein bands will be visible after the proteins are transferred to a blot, probed with myosin antibody, and subjected to colorimetric detection.


a. This statement is incorrect because the polyacrylamide gel electrophoresis (PAGE) separates all proteins in the sample, not just myosin. Therefore, each lane will contain multiple bands representing various proteins in the muscle tissue.

b. This statement is correct because PAGE separates proteins based on their size and charge, leading to multiple blue bands in each lane. Coomassie blue stains all proteins, so it will display an array of proteins present in fish muscle tissue.

c. This statement is incorrect because although Western blotting is a technique used to detect specific proteins (like myosin), the question states that the gel is stained with Coomassie blue, which stains all proteins.

d. This statement is correct because if the proteins are transferred to a blot and probed with an antibody specific for myosin, only the myosin bands will be detected through colorimetric detection. This method is called Western blotting and is used to detect and analyze specific proteins within a sample.

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Related Questions

what is the function of mitosis?​

Answers

Answer:

Mitosis is a process where a single cell divides into two identical daughter cells (cell division). During mitosis one cell? divides once to form two identical cells. The major purpose of mitosis is for growth and to replace worn out cells.

Explanation:

Hope it is helpful....

What is bacterial conjugation?

Answers

Answer:

Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge-like connection between two cells. This takes place through a pilus. It is a parasexual mode of reproduction in bacteria

Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge-like connection between two cells. This takes place through a pilus. It is a parasexual mode of reproduction in bacteria.

6. How does biogeography explain how anteaters evolved on many continents?

Answers

Biogeography, the study of the geographical distribution of organisms, provides information about how and when species may have evolved. Fossils provide evidence of long-term evolutionary changes, documenting the past existence of species that are now extinct.

8.What attaches muscles to bones?

veins
tendons
ligaments
joints

Answers

Tenons attach the muscles to the bones

Twenty years after his voyage to the Galapagos, what did Darwin identify as the creative force driving the evolution of species?

Answers

Darwin discovered many animals but he observed finches(a type of bird) with unique beaks.

Natural selection drives evolution

Which process involves starting with a DNA message and ending with mRNA?
a. replication
b. transcription
c. translation
d. translocation

Answers

I think its c. Translation
B because the mRNA is like a transcript or copy of the gene’s DNA code.

What percentage of those struck by
lightning, live to tell about it?

Answers

of every ten people struck by lightning, nine will survive and tell the tale.

What leads to variation in a population?​

Answers

Answer:

Genetic variation can be caused by mutation (which can create entirely new alleles in a population), random mating, random fertilization, and recombination between homologous chromosomes during meiosis (which reshuffles alleles within an organism's offspring).

Explanation:

Why is clay like a stem cell? *

a. Clay is made of particles just like a stem cell

b. Clay can be made into anything (like a cup or a figurine) just like a stem cell can become different types of cells

c. Clay is not at all like a stem cell

Answers

Answer:

B. Clay can be made into anything (like a cup or a figurine) just like a stem cell can become different types of cells

Explanation:

The nucleus acid blank copies DNA so blank can be made

Answers

Answer:

The nucleus acid Combinent copies DNA so Rna can be made

inherited traits are governed controlled by

Answers

Answer:

Inherited traits are controlled by genes. In this case, genetic material.
Inherited traits are controlled by genes and the complete set of genes within an organism's genome is called its genotype.

Genetic material.

Which is NOT a part of a bivalve?
A. mantle
B. mouth
C. radula
D. shell

Answers

Answer:

radula

Explanation:

The picture below shows four locations and their temperatures. The temperature in each location is given in Celsius. Answer the question using what you know about heat transfer and how it ALWAYS travels.

Answers

Answer:

en español por favor

How many electrons surround the
nucleus of an atom of Carbon?

Answers

Answer:

it has 2 atoms of carbon

Explanation:

Answer:

There are 6 electrons in a carbon atom.

Explanation:

Miguel y Andrés empujan dos cajas del mismo material y de diferente peso, aplicándoles la misma fuerza, como se muestra en la siguiente figura: Si Miguel y Andrés empiezan a empujar sus cajas al mismo tiempo, ¿cuál de los dos cruzará primeroel banderín?

Answers

Answer:

Cualquiera sea más ligero porque será más fácil de empujar. Dando como resultado la caja para superar la línea de meta más rápido

Explanation:

air passes from the nose into the bronchi true or false?

Answers

Answer:

True

Explanation:

The right lung has 3 sections called lobes and is a little larger than the left lung, which has 2 lobes. The bronchial tubes divide into smaller air passages called bronchi. The functioning of the lungs is discussed in detail.

Why is writing on word better than writing on a sheet of paper?

Answers

Answer:

because on paper your hand wrighting is bigger and you go through more sheets and on word it has auto correct and auto save and suggestions

Explanation:

can i have brainlyest

Answer:

Writing in Word is more convenient

Explanation:

Word has a host of text, correction software it suggests proper grammer and spelling and can format your doucument.

how would you make an extract of a food to test for reducing sugar​

Answers

To test for the presence of reducing sugars, a food sample is dissolved in boiling water. Next, a small amount of Benedict's reagent is added and the solution begins to cool. During the next four to 10 minutes, the solution should begin to change colors. If the color changes to blue, then no glucose is present. If a high amount of glucose is present, then the color change will progress to green, yellow, orange, red and then a dark red or brown.

I need help with 1,2,4,5,6, and 7. I already did 3 :)

Answers

Answer:

Answers, in order:

40, Temperature steeply decreases, 2, Effect decreases steeply, 5 and 9, 10


Which organelle is the location where mRNA is translated into a protein?

Answers

Answer:

its ribosomes. In eukaryotes, mature mRNA molecules must leave the nucleus and travel to the cytoplasm, where the ribosomes are located.

Answer:

ribosomes

Explanation:

‼️‼️‼️‼️‼️
which population is most likely to increase as a result?
A. deer
B. mouse
C. owl
D. Hawk

Answers

Answer:

A. Deer

Explanation:

cause only mountain lions eat deers. without them theyll increase.

I think it would be d , hawks

Can you please help me?!❤️

Answers

Answer:

A. Strainer

Explanation:

A strainer is, amongst other things, something used to wash foods like fruits, vegetables, &tc and rinse out certain foods, like rice.

What happens when you put something through a strainer? When you wash something with it, the dirt goes through but not the food, like in the image, right?

Have a great day!

PICK ONE ONLY FOR EACH ONE
1) harry potter, maze runner, divergent
2) blue, black, purple
3) Football , Basketball, soccer
4) pizza , tacos , soup

Answers

Answer:

divergent, black, football, tacos

Explanation:

Tommy’s dad is teaching him how to make tables out of tiles. Tommy makes a small table that is 3 feet wide and 4 feet long. How many square foot tiles does he need to cover the top of the table? How many feet of decorative border material will his dad need to cover the edges of the table? I need area and perimeter

Answers

Answer:

pues búscalo en internet y vas enserar lo más importantes

Area=12
Perimeter=14

Sister chromatids are ____.. Single choice.

A. dense patches within the nucleus
B. bacterial chromosomes
C. joined strands of duplicated genetic material
D. prokaryotic nuclei
Option 2

Please hellppppp!

Answers

the answer is C i hope it’s right but i searched up

Answer:

C- joined strands of duplicated genetic material

Explanation:

key words duplicated sister

Need help

The photograph shows a consequence of air pollution.
Which of these was most likely the cause of the effect shown in the
photograph?

A. Eutrophication

B. Acid deposition

C. Ozone depletion

D. Bioremediation

Answers

The most likely cause of the effect shown in the given photograph is Acid deposition.

What are the causes of Air pollution?

The causes of air pollution include excessive burning of fossil fuels, industrial emissions, carbon emissions from automobiles, construction and industrial wastes, etc.

Acid deposition may be also known as Acid rain. It includes the form of precipitation with several acidic components like nitric acid and sulfuric acid.

These precipitations finally falls on the earth from the atmosphere in the form of rain, snow, fog, etc., and are deposited on tall trees, buildings, and objects, and lose their natural beauty.

Therefore, the most likely cause of the effect shown in the given photograph is Acid deposition.

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Answer:

Acid Depletion

Explanation:

How can a material at a certain temperature have all of its molecules at the same energy?

Answers

it can be frozen (32°F or 0°C) which would slow the energy of the molecules. or it could be boiled (212°F or 100°C) which would rapidly increase the speed of the molecules.

Recessive traits are...


visible in the organism's phenotype as long as TWO recessive traits are present.


visible in the organism's phenotype as long as ONE recessive trait is present.

Answers

I don’t really understand this question can you go more into detail

how does understanding cell reproduction allow scientists to generate organs and tissues in the laboratory.

Answers

It can allow them to start creating clones. And with clones, it could help delay the extinction of certain species.

In a herd of beef cattle, the average yearling weight of all animals was 800 lb when corrected to a bull basis. Bulls and heifers kept for breeding weighed 1000 and 825 lb, respectively. The progeny of those individuals selected for breeding had an average yearling weight corrected to a bull basis of 845 lb. What is the apparent genetic control of the trait

Answers

Answer:

Explanation:

The trait is genetic and not dependent on environment. When a trait is dependent on the genetic component of the parental generation, the ability of the traits to be passed from a generation to another is high and this will be revealed in their resemblance with the parent. Hence, the case of the offspring with an average weight of 845lb.

If the trait is controlled or influence by the environment there will be a difference when the offspring is produce because until the same environment is given to the offspring the trait cannot be expressed.

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