Digestion of lambda DNA with Eco RI generated 6 fragments. The Pst I restriction enzyme produces 29 lambda DNA fragments.
What are restriction enzymes?Restriction enzymes are DNA-cutting enzymes found in bacteria. These are site-specific endonucleases also known as molecular scissors.These recognize and cut DNA only at a particular sequence of nucleotides.DNA from the bacteriophage Lambda (48,502 base pairs in length) is cut with a variety of restriction enzymes and the resulting fragments are separated using gel electrophoresis. Size of fragment of uncut lambda DNA is 48502 b.p.Eco RI recognizes G AATTC
CTTAA G
Lambda phage has 5 recogition sites for Eco RI. It cleaves the DNA at ‘G’ of the above sequence.Relative sites of cleavage in base pairs are 21226, 26104, 31747, 39168, 44972.Sizes of fragments produced when the lambda DNA cut with Eco RI 21226, 7421, 5804, 5643, 4878, 3530.Digestion of lambda DNA with EcoRI generated 6 fragments but 2 of them are so close in size that they are difficult to separate.Pst I recognizes CTGCA G
G ACGTC
The Pst I restriction enzyme produces 29 fragments of lambda DNA. It cleaves the DNA at ‘G’ of the above sequence.The Pst I digest of λ DNA yields the following discrete fragments (in base pairs): 11497, 5077, 4749, 4507, 2838, 2556, 2459, 2443, 2140, 1986, 1700, 1159 1093, 805, 514, 468, 448, 339, 264, 247, 216, 211, 200, 164, 150, 94, 87, 72 and 15.The cohesive ends (12b cos site of bacteriophage) of fragments 11501 bp and 2556 bp may anneal and form an additional band.Learn more about restriction enzymes here:
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discuss how classification system have undergone
Answer:
The classification systems have undergone several changes with time. The first attempt of classification was made by Aristotle. He classified plants as herbs, shrubs, and trees. Animals, on the other hand, were classified on the basis of presence or absence of red blood cells.
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Where would you least
likely find a new
volcano?
A. Mid-ocean ridge
B. "Hot spot" in Hawaii
C. The great plains in the middle
of the U.S.
D. Near a subduction zone in
Argentina
Secon2015
I NEED TO KNOW ASAP
Answer:
A. Mid-ocean ridge. I'm not sure if I'm right.
The place where the new volcanoes are less likely to be seen is mid-ocean ridge. The correct option is A.
What is volcano?A volcano is a crack in the crust of a planet, such as Earth, that allows hot lava, volcanic ash, and gases to escape from a magma chamber beneath the surface.
Volcanoes are most commonly found where tectonic plates are diverging or converging on Earth, and the majority are found underwater.
Although Australia is the only continent with no active volcanoes, it does have one of the world's largest extinct volcanoes, the Tweed Volcano.
Volcanism is directly related to plate tectonics theory. Volcanoes are only found at specific types of plate boundaries, and only rarely in the middle of ocean plates.
Thus, the correct option is A.
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Subduction zones occur along
a. divergent boundaries.
b. convergent boundaries.
c. transform boundaries.
d. in the middle of plates, above hot spots.
Answer:
B, porque es la mejor respuesta de la pregunta
ILL GIVE YOU BRAINLYIST
Answer:
d
D
D
D
D
D
Explanation:
DD
D
D
D
D
D
Can someone please help me please
Answer:
I think so answer is D
Explanation:
I hope it helps you
Pl Mark brainlist answer
What is a simple diffusion?
Answer:
movement of a solute from an area of high concentration to an area of low concentration
Explanation:
Which gland is located closest to the human brain?
A The thyroid
B The pituitary
C The adrenal
D The ovaries
Answer:
B. The pituitary gland
Explanation:
Can't be the ovaries, they're down by the hips!
Can't be adrenal glands, they're on top of the kidneys!
Can't be the thyroid, that's low on the front of the neck or something like that!
It's B, the pituitary; it's on the base of the brain.
Have a great day!
Mitosis and budding are similar beu
O
Both processes are components of sexual reproduction
The offspring produced by both are genetically diverse
In both, the genetic material comes from a single parent.
O O
Both processes are more common in animals than in plants.
Answer:
animals would be your awnser you are welcome
Explanation:
keep it cool ansd answer my question if u can! :))
Which configuration and conditions helped the perfume molecules diffuse better?
Answer:
Higher temperatures increase the energy and therefore the movement of the molecules, increasing the rate of diffusion
Explanation:
As the density of a solvent increases, the rate of diffusion decreases. yep i know
How do environmental issues impact food production?
Answer:
hola esta en ingles alguien sabe como ponerlo em español
What peppery of water allows it to move up through a plant?
Answer:
A. Hydrogen Bonds
How do our social groups and social interactions impact our behavior?
Answer:
it makes us addict to fame and people would do anything to be higher up in the social groups
A mouse are 380 calories worth of cherries. A fox catches it . How many calories does the fox obtain?
Answer:
The correct answer would be - 38 calories.
Explanation:
True or False
a. The sinus node generates an electrical impulse.
b. The bundle of His transfers the impulse to the AV node.
c. The fiber group stimulates only the right ventricles.
d. The cardiac cycle represents one complete heartbeat.
e. Relaxation events are referred to as diastole.
Answer:
Explanation:
A-true
B-false
C-false
D-true
E- true
____is a restriction to the flow of electrons
Answer:
Impedance.
Explanation:
Conductors allows the flow of electrons and Impedance restricts it.
2 differences between
monocotyledondus and dicotyledonas plants
Answer:
1)The monocot embryos have a single cotyledon.
The dicot embryos have a pair of cotyledons.
monocotyledons
2)They have a fibrous root system.
dicotyledonous.
They have a tap root system.
Type the correct answer in the box. Spell all words correctly.
Which gas prevents the spread of fire and extinguishes it?
Blank. is a type of compressed gas that prevents the spread of fire and extinguishes it.
Help ASAP !!!
Answer:
Halon
Explanation:
I got it correct on Plato
process of gathering information about events or processes in an orderly way
Answer:
Observation is the process of gathering information about events or processes in an orderly way
7. A man sitting outside a restaurant on a park bench is smoking a cigarette while reading a newspaper. Which answer best describes the mutagen to which the man is exposed?
O The newspaper
The cigarette
The oxygen he is breathing
The park bench
what would probably happen if demario shared a drinking glass with his best friend who was sick with the flu and had a fever
Answer:
Demario would probably get the flu as well
Explanation:
Which of the following is a macronutrient?
A) iron B) boron C) Calcium D) Manganese
PLEASE HELP!!!!! I NEED PERSON GOOD WITH AP BIOLOGY!! I WILL GIVE BRAINLIEST PLS!!!
Answer:
if crossing over does not occur, the products are parental gametes. If crossing over occurs, the products are recombinant gametes. Meaning they are mixed.
Explanation:
If crossing over doesn't occurs then you wouldn't really mix the 2 parental alleles so you couldn't have a genotype without mixing.
Sorry if im wrong!
2. Codominance: Diricrawls are plump, fluffy-feathered, flightless birds. Feather color
is co-dominant; FF have pink feathers, PP have purple feathers and mottled diriclaws (FP)
have both pink and purple feathers. Pink feathered diricrawls are very rare and prized by
collectors. If you breed a pink diriclaw with a mottled diricrawls, what percentage of their
offspring will be pink feathered? (show all of your work and use a Punnett square).
Answer:
The correct answer is - 50% or 1/2.
Explanation:
In codominance both alleles express their traits and blend to make new traits, there is no dominance or recessive pattern found. In this question, there is FF have pink feathers, PP has purple feathers and mottled diriclaws (FP). Then a cross between pink feathers and mottled diriclaws would produce offspring:
F F
F FF FF
P FP FP
Here two out of four have pink feathers. That is 50%
PLS HELP ILL MARK BRAILIEST
Spend one minute writing an explanation of
the FUNCTION of enzymes. YOU MUST USE
THE WORDS CATALYST, ACTIVATION
ENERGY, RECYCLABLE
Answer:
Enzymes help speed up chemical reactions in the human body. They bind to molecules and alter them in specific ways. They are essential for respiration, digesting food, muscle and nerve function, among thousands of other roles.
What is the function of an enzyme? They allow chemical reactions to occur at normal body temperature fast enough to sustain life. They reduce the activation energy needed to start a chemical reaction.
There are four steps in the process of an enzyme working. (1) An enzyme and a SUBSTRATE are in the same area. The substrate is the biological molecule that the enzyme will work on. (2) The enzyme grabs onto the substrate with a special area called the ACTIVE SITE.
There are four steps in the process of an enzyme working. (1) An enzyme and a SUBSTRATE are in the same area. The substrate is the biological molecule that the enzyme will work on. (2) The enzyme grabs onto the substrate with a special area called the ACTIVE SITE.
Enzymes create chemical reactions in the body. They actually speed up the rate of a chemical reaction to help support life. The enzymes in your body help to perform very important tasks. These include building muscle, destroying toxins, and breaking down food particles during digestion.
Which of the following statements is NOT part of the cell theory? a All cells originate from other cells. b The most basic component of any organism is the cell. c All living things are made up of one or more cells. d All cells have a nucleus and a cell membrane.
Answer:
d) All cells have a nucleus and a cell membrane.
Explanation:
Prokaryotes do not have a nucleus
If the sphincter allows any leakage from the stomach, some of this acid might move back into the esophagus, causing
1. Heartburn
2. Saliva
3. Constipation
4. Diarrhea
Are physical characteristics of an organism not genetically passed down?
why can it take soil thousands of years to form
design your own, you can use the Browse feature to attach and upload your document. 5. Design your own experiment based on one of your questions from question 1, above. Include the question, hypothesis, independent and dependent variables, and safety precautions. 6. What are three major branches of Earth science?
Answer:
three major branches of earth science are Geology, oceanography, meteorology.